Multi-walled carbon nanotubes/carbon black/rPLA for high-performance conductive additive manufacturing filament and the simultaneous detection of acetaminophen and phenylephrine.

The combination of multi-walled carbon nanotubes (MWCNT) and carbon black (CB) is presented to produce a high-performance electrically conductive recycled additive manufacturing filament. The filament and subsequent additively manufactured electrodes were characterised by TGA, XPS, Raman, and SEM and showed excellent low-temperature flexibility. The MWCNT/CB filament exhibited an improved electrochemical performance compared to an identical in-house produced bespoke filament using only CB. A heterogeneous electrochemical rate constant, [Formula: see text] of 1.71 (± 0.19) × 10-3 cm s-1 was obtained, showing an almost six times improvement over the commonly used commercial conductive CB/PLA. The filament was successfully tested for the simultaneous determination of acetaminophen and phenylephrine, producing linear ranges of 5-60 and 5-200 µM, sensitivities of 0.05 µA µM-1 and 0.14 µA µM-1, and limits of detection of 0.04 µM and 0.38 µM, respectively. A print-at-home device is presented where a removable lid comprised of rPLA can be placed onto a drinking vessel and the working, counter, and reference components made from our bespoke MWCNT/CB filament. The print-at-home device was successfully used to determine both compounds within real pharmaceutical products, with recoveries between 87 and 120% over a range of three real samples. This work paves the way for fabricating new highly conductive filaments using a combination of carbon materials with different morphologies and physicochemical properties and their application to produce additively manufactured electrodes with greatly improved electrochemical performance.

The use of biological fluids in microfluidic paper-based analytical devices (µPADs): Recent advances, challenges and future perspectives.

The use of microfluidic paper-based analytical devices (µPADs) for aiding medical diagnosis is a growing trend in the literature mainly due to their low cost, easy use, simple manufacturing, and great potential for application in low-resource settings. Many important biomarkers (proteins, ions, lipids, hormones, DNA, RNA, drugs, whole cells, and more) and biofluids are available for precise detection and diagnosis. We have reviewed the advances µPADs in medical diagnostics have achieved in the last few years, focusing on the most common human biofluids (whole blood/plasma, sweat, urine, tears, and saliva). The challenges of detecting specific biomarkers in each sample are discussed, along with innovative techniques that overcome such limitations. Finally, the difficulties of commercializing µPADs are considered, and future trends are presented, including wearable devices and integrating multiple steps in a single platform.

Autosomal dominant polycystic kidney disease in Colombia.

BACKGROUND: Autosomal dominant polycystic kidney disease (ADPKD) is the most common genetic cause of chronic kidney disease (CKD) that requires dialysis. Knowing geographical clusters can be critical for early diagnosis, progression control, and genetic counseling. The objective was to establish the prevalence, geographic location, and ethnic groups of patients with ADPKD who underwent dialysis or kidney transplant in Colombia between 2015 and 2019. METHODS: We did a cross-sectional study with data from the National Registry of Chronic Kidney Disease (NRCKD) managed by the High-Cost Diseases Fund (Cuenta de Alto Costo [CAC] in Spanish) between July 1, 2015, and June 30, 2019. We included Colombian population with CKD with or without renal replacement therapy (RRT) due to ADPKD. Crude and adjusted prevalence rates were estimated by state and city. RESULTS: 3,339 patients with ADPKD were included, period prevalence was 9.81 per 100,000 population; there were 4.35 cases of RRT per 100,000 population, mean age of 52.58 years (± 13.21), and 52.78% women. Seventy-six patients were Afro-Colombians, six were indigenous, and one Roma people. A total of 46.07% began scheduled dialysis. The highest adjusted prevalence rate was in Valle del Cauca (6.55 cases per 100,000 population), followed by Risaralda, and La Guajira. Regarding cities, Cali had the highest prevalence rate (9.38 cases per 100,000 population), followed by Pasto, Medellin, and Bucaramanga. CONCLUSIONS: ADPKD prevalence is lower compared to Europe and US; some states with higher prevalence could be objective to genetic prevalence study.

Carbon Nanomaterials-Based Screen-Printed Electrodes for Sensing Applications.

Electrochemical sensors consisting of screen-printed electrodes (SPEs) are recurrent devices in the recent literature for applications in different fields of interest and contribute to the expanding electroanalytical chemistry field. This is due to inherent characteristics that can be better (or only) achieved with the use of SPEs, including miniaturization, cost reduction, lower sample consumption, compatibility with portable equipment, and disposability. SPEs are also quite versatile; they can be manufactured using different formulations of conductive inks and substrates, and are of varied designs. Naturally, the analytical performance of SPEs is directly affected by the quality of the material used for printing and modifying the electrodes. In this sense, the most varied carbon nanomaterials have been explored for the preparation and modification of SPEs, providing devices with an enhanced electrochemical response and greater sensitivity, in addition to functionalized surfaces that can immobilize biological agents for the manufacture of biosensors. Considering the relevance and timeliness of the topic, this review aimed to provide an overview of the current scenario of the use of carbonaceous nanomaterials in the context of making electrochemical SPE sensors, from which different approaches will be presented, exploring materials traditionally investigated in electrochemistry, such as graphene, carbon nanotubes, carbon black, and those more recently investigated for this (carbon quantum dots, graphitic carbon nitride, and biochar). Perspectives on the use and expansion of these devices are also considered.

Using low-cost disposable immunosensor based on flexible PET screen-printed electrode modified with carbon black and gold nanoparticles for sensitive detection of SARS-CoV-2.

To help meet the global demand for reliable and inexpensive COVID-19 testing and environmental analysis of SARS-CoV-2, the present work reports the development and application of a highly efficient disposable electrochemical immunosensor for the detection of SARS-CoV-2 in clinical and environmental matrices. The sensor developed is composed of a screen-printed electrode (SPE) array which was constructed using conductive carbon ink printed on polyethylene terephthalate (PET) substrate made from disposable soft drink bottles. The recognition site (Spike S1 Antibody (anti-SP Ab)) was covalently immobilized on the working electrode surface, which was effectively modified with carbon black (CB) and gold nanoparticles (AuNPs). The immunosensing material was subjected to a multi-technique characterization analysis using X-ray diffraction (XRD), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) with elemental analysis via energy dispersive spectroscopy (EDS). The electrochemical characterization of the electrode surface and analytical measurements were performed using cyclic voltammetry (CV) and square-wave voltammetry (SWV). The immunosensor was easily applied for the conduct of rapid diagnoses or accurate quantitative environmental analyses by setting the incubation period to 10 min or 120 min. Under optimized conditions, the biosensor presented limits of detection (LODs) of 101 fg mL-1 and 46.2 fg mL-1 for 10 min and 120 min incubation periods, respectively; in addition, the sensor was successfully applied for SARS-CoV-2 detection and quantification in clinical and environmental samples. Considering the costs of all the raw materials required for manufacturing 200 units of the AuNP-CB/PET-SPE immunosensor, the production cost per unit is 0.29 USD.

Development of New Simple Compositions of Silver Inks for the Preparation of Pseudo-Reference Electrodes.

Silver materials are known to present excellent properties, such as high electrical and thermal conductivity as well as chemical stability. Silver-based inks have drawn a lot of attention for being compatible with various substrates, which can be used in the production uniform and stable pseudo-reference electrodes with low curing temperatures. Furthermore, the interest in the use of disposable electrodes has been increasing due to the low cost and the possibility of their use in point-of-care and point-of-need situations. Thus, in this work, two new inks were developed using Ag as conductive material and colorless polymers (nail polish (NP) and shellac (SL)), and applied to different substrates (screen-printed electrodes, acetate sheets, and 3D-printed electrodes) to verify the performance of the proposed inks. Measurements attained with open circuit potential (OCP) attested to the stability of the potential of the pseudo-reference proposed for 1 h. Analytical curves for ß-estradiol were also obtained using the devices prepared with the proposed inks as pseudo-references electrodes, which presented satisfactory results concerning the potential stability (RSD < 2.6%). These inks are simple to prepare and present great alternatives for the development of pseudo-reference electrodes useful in the construction of disposable electrochemical systems.

Grapiprant or carprofen following ovariohysterectomy in the cat: analgesic efficacy, hematological, biochemical and urinalysis evaluation.

OBJECTIVES: This study aimed to compare the analgesic effect between carprofen and grapiprant every 12 or 24 h on postoperative pain in cats undergoing ovariohysterectomy, in addition to the effects on the hematological, biochemical and urinalysis variables. METHODS: A total of 32 female cats were randomly divided into three groups, according to the treatment administered with the first dose given orally 90 mins before surgery, as follows: CAR (cats received 4 mg/kg carprofen, n = 11); GRA1 (cats received 2 mg/kg grapiprant, n = 10); and GRA2 (cats received 2 mg/kg grapiprant q12h, n = 11). Pain was assessed by UNESP-Botucatu Multidimensional Composite Pain Scale (UNESP) and Glasgow Feline Composite Measure Pain Scale (GLASGOW) for cats preoperatively (baseline) and at 1, 3, 6, 8, 12 and 24 h after extubation. Venous blood was collected at baseline, and 12 and 24 h after the administration of carprofen or grapiprant to perform a complete blood count (CBC), the percentage of Heinz bodies and serum biochemistry (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma-glutamyltransferase, creatinine and urea). Urinalysis was performed at baseline and 24 h after extubation. Glucose levels were evaluated at baseline and 1 h postoperatively. RESULTS: Pain scores were not significantly different among groups in both scales, although pain was higher at 3 h in comparison with 24 h in all groups. In the GRA1 and GRA2 groups, 67% (14/21) of cats needed rescue analgesia compared with 18% (2/11) in the CAR group. Glucose increased from baseline to 1 h in the GRA1 and GRA2 groups. None of the CBC, serum biochemistry and urinalysis variables differed among groups. CONCLUSIONS AND RELEVANCE: Grapiprant did not promote adequate analgesia during the first 3 h postoperatively in cats undergoing ovariohysterectomy compared with carprofen, and no benefits were observed by administering grapiprant every 12 h.

Laryngeal Paralysis Following Endotracheal Intubation in a Dog: A Case Report.

The occurrence of laryngeal paralysis following endotracheal intubation in dogs is rare. A one-year-old canine was presented with aspiration pneumonia in the postoperative period following general anesthesia for acetabular denervation surgery. After a month of treatment for aspiration pneumonia, the patient had inspiratory stridor and dyspnea, and the diagnosis of unilateral laryngeal paralysis was made through laryngoscopy. Along with the benefits of endotracheal intubation come many risks. Laryngeal paralysis can be a serious complication, predisposing the patient to aspiration. This report is the second in veterinary medicine to describe laryngeal paralysis as a possible complication after endotracheal intubation in a dog.

Intervenção para mães com depressão pós-parto: protocolos de psicoeducação e treino para reconhecimento de emoção / Intervention for mothers with postpartum depression: psychoeducation and training for emotion recognition protocols / Intervención para madres con depresión posparto: protocolos de psicoeducación y capacitación para el reconocimiento de la emoción

A Depressão Pós-Parto Materna (DPP) é uma condição clínica que acarreta efeitos negativos na saúde da mãe, do bebê, e na relação entre ambos. Intervenções neste contexto devem ser adaptadas à realidade, a fim de diminuir os impactos no desenvolvimento infantil e na saúde materna. O objetivo deste estudo foi a construção de um protocolo de psicoeducação e a elaboração de um treinamento de reconhecimento de emoções. O método se constituiu de quatro etapas: revisão da literatura, proposta preliminar, análise de especialistas e estudo piloto. A psicoeducação foi guiada por sete cartilhas com temáticas típicas do desenvolvimento, e o treinamento de reconhecimento de emoções foi composto por um protocolo de atendimento para mães com DPP e seus bebês, com o objetivo de trabalhar questões relativas ao processamento de faces materno e viés atencional. Os resultados demonstraram que as duas propostas de protocolo de intervenção se mostraram de fácil compreensão para os sujeitos avaliados.

Postpartum Maternal Depression (PPD) is a clinical condition that has negative effects on the health of the mother, the baby and the relationship between both. Interventions in this context must be adapted to reality in order to reduce the impacts on child development and maternal health. The aim of this study was to build a psychoeducational protocol and to develop an emotion recognition training. The method consisted of four stages: literature review, preliminary proposal, expert analysis and pilot study. Psychoeducation was guided by seven booklets with themes typical of development, and the emotion recognition training consisted of a care protocol for mothers with PPD and their babies, with the objective of working on issues related to the processing of maternal faces and attentional bias. The results showed that the two intervention protocol proposals proved to be easy to understand for the evaluated subjects.

La depresión materna posparto (DPP) es una condición clínica que tiene efectos negativos en la salud de la madre, el bebé y la relación entre ambos. Las intervenciones en este contexto deben adaptarse a la realidad para reducir los impactos sobre el desarrollo infantil y la salud materna. El objetivo de este estudio fue construir un protocolo psicoeducativo y desarrollar un entrenamiento de reconocimiento de emociones. El método consistió en cuatro etapas: revisión de la literatura, propuesta preliminar, análisis de expertos y estudio piloto. La psicoeducación se guió por siete cartillas con temas típicos del desarrollo, y el entrenamiento de reconocimiento de emociones consistió en un protocolo de atención para madres con DPP y sus bebés, con el objetivo de trabajar en temas relacionados con el procesamiento de rostros maternos y sesgos atencionales. Los resultados mostraron que las dos propuestas de protocolo de intervención demostraron ser de fácil comprensión para los sujetos evaluados.

Histopathological Classifications of Oral Leukoplakia and its Relation to Cell Proliferative Activity: A Case Series.

AIM: This study relates the average number of nucleolar organizing regions (NORs) obtained in a series of cases of oral leukoplakia, with three methods of histopathological classification of such lesion. MATERIALS AND METHODS: This is a histopathological-histochemical laboratory cross-sectional study. The 18 cases of leukoplakia analyzed were filed at Pathology Service of the Biological Sciences Institute of the University of Passo Fundo, Rio Grande do Sul, Brazil (SDH/ICB/UPF) (2017 and 2018), from which epidemiological data were extracted. New histological sections were performed for impregnation by the argyrophilic nucleolar organizer regions (AgNOR) technique. The histopathological slides were analyzed by photon microscopy (1,000×), and the nuclei of 100 epithelial cells were photographed to count the number of NORs. Three methods were used for the lesions' histopathological classification [World Health Organization (WHO), Brothwell, and binary system]. The means of NORs were compared with the three histopathological classifications by means of the t or analysis of variance (ANOVA) statistical tests, at a significance level of 5%. RESULTS: According to the WHO classification method, most cases (11-61.1%) had a moderate classification. Evaluations by the Brothwell method showed moderate and mild classification in 50 and 38.9% of cases, respectively. According to the binary system, most cases (10-55.6%) had low risk. The average NORs found in 100 nuclei of each of the 18 lesions ranged from 2 to 4. When crossing the average NORs with the histopathological classification methods of the lesions by means of the t test or ANOVA, no significant relationship was noted. CONCLUSION: The average of NORs is not associated with the histological classifications of leukoplakias. Thus, the AgNOR method should be used with caution when differentiating different histological grades of leukoplakias. CLINICAL SIGNIFICANCE: The AgNOR method should be used with caution to determine the clinical treatment of oral leukoplakias, since no agreement was observed between this method and the histopathological classifications available for such lesion.

Yellow fever surveillance challenge: Investigation of a marmoset non-autochthonous case.

Yellow fever (YF) surveillance in Brazil is focused mainly on the detection of epizootic events regarding New World non-human primates (NWNHP). We present a challenging case of a Callitrichidae (Callithrix spp) kept as a domiciliated pet that lived in the urban area of São Paulo municipality and was positive to YF virus by RT-qPCR and immunohistochemistry. After investigation, it was the first occurrence of non-autochthonous YF case of NWNHP described, with probable place of infection in the North shore of São Paulo state. This case illustrates the importance of coordinated laboratorial and field actions, and risks posed by transit of wildlife.

Waterproof paper as a new substrate to construct a disposable sensor for the electrochemical determination of paracetamol and melatonin.

Disposable electrochemical sensors using sustainable and cheap materials are an exciting alternative to produce new kinds of sensing platforms. Waterproof paper (WP) is a biodegradable and biocompatible material that allows dropped of the sample on its surface without absorption by fibers. Also, WP can be used for miniaturized sensors construction. In this work, a conductive ink was produced with nail polish and graphite powder, using the WP as the sensor substrate for paracetamol (PAR) and melatonin (MEL) voltammetric determination. PAR is a pharmaceutical commonly used in high doses for the relief of pain and fever, and MEL is a hormone related to several diseases besides a direct relation to sleep quality. Using differential pulse voltammetry for PAR determination, the WP sensor showed a linear response in the concentration ranging from 0.50 µmol L-1 to 100 µmol L-1 with a limit of detection (LOD) of 53.6 nmol L-1. Square wave voltammetry was applied for MEL determination, and the proposed electrode presented linear response ranging from 0.80 µmol L-1 to 100 µmol L-1 and LOD of 32.5 nmol L-1. The sensor showed excellent repeatability and reproducibility for consecutive measurements. Then, the disposable WP sensor was successfully applied in the determination of PAR and MEL in pharmaceutical and biological samples, with recovery values, above 91.1%. The described architecture allowed the manufacture of a disposable, simple, and low-cost electroanalytical device that can be used for electrochemical sensing.

Yellow fever surveillance challenge: Investigation of a marmoset non-autochthonous case

Yellow fever (YF) surveillance in Brazil is focused mainly on the detection of epizootic events regarding New World non-human primates (NWNHP). We present a challenging case of a Callitrichidae (Callithrix spp) kept as a domiciliated pet that lived in the urban area of São Paulo municipality and was positive to YF virus by RT-qPCR and immunohistochemistry. After investigation, it was the first occurrence of non-autochthonous YF case of NWNHP described, with probable place of infection in the North shore of São Paulo state. This case illustrates the importance of coordinated laboratorial and field actions, and risks posed by transit of wildlife.

Mobilidade funcional de pacientes críticos em terapia intensiva: um estudo piloto / Functional mobility of critical patients in intensive care: a pilot study

Objetivo ­Avaliar e comparar a mobilidade funcional de pacientes críticos internados em unidade de terapia intensiva (UTI) na admissão e na alta da unidade. Métodos ­ Estudo piloto realizado na UTI Geral e UTI Neurocirúrgica de um hospital escola, em São José do Rio Preto, SP. Foram selecionados prospectivamente pacientes adultos entre 18 e 60 anos, sedados, em ventilação mecânica invasiva. Foram identificados dados sociodemográficos, clínicos e hemodinâmicos à admissão na unidade e aplicada a escala de mobilidade funcional em UTI (EMU), na admissão e na alta da unidade. Foi aplicada estatística inferencial com teste de Wilcoxon. O nível de significância foi p ≤ 0,05. Resultados ­ A amostra foi composta por 28 pacientes, maior prevalência (54%) do sexo masculino, com média de idade de 54,56±22,52 anos. A especialidade de internação mais prevalente foi a neurológica com 57%. Houve incremento significativo (p <0,0001) na comparação da mobilidade funcional entre admissão (0,25 ± 0,44) e alta (2,21 ± 1,77) da UTI. Conclusão ­ Essa amostra de pacientes críticos, internados em unidade de terapia intensiva sedados e em ventilação mecânica invasiva, evoluiu com melhora estatística significativa da mobilidade funcional na comparação entre a admissão e a alta da unidade. Contudo, em termos clínicos, não houve evolução na funcionalidade desta amostra de pacientes da admissão para a alta da UT

Objective ­ To evaluate and compare the functional mobility of critically ill patients admitted to an intensive care unit (ICU) on admission and discharge. Methods ­ A pilot study performed at the General ICU and Neurosurgical ICU of a school hospital in São José do Rio Preto, SP. We prospectively selected adult patients between 18 and 60 years of age, sedated, on invasive mechanical ventilation. Sociodemographic, clinical and hemodynamic data were identified at admission to the unit and the functional mobility scale at ICU (EMU), admission and discharge at the unit were applied. Inferential statistics were used with Wilcoxon test. The level of significance was p ≤ 0.05. Results ­ The sample consisted of 28 patients, a higher prevalence (54%) of males, with a mean age of 54.56 ± 22.52 years. The most prevalent hospitalization specialty was neurological with 57%. There was a significant (p <0.0001) increase in functional mobility between admission (0.25 ± 0.44) and high (2.21 ± 1.77) ICU. Conclusion ­ This sample of critically ill patients hospitalized in a sedated intensive care unit and in invasive mechanical ventilation evolved with a statistically significant improvement in functional mobility in the comparison between admission and discharge. However, in clinical terms, there was no evolution in thefunctionality of this sample of patients from admission to discharge from the ICU

Hanseníase e trabalho: a correlação da doença com o afastamento do trabalhador em tratamento na Unidade Básica de Saúde / LEPROSY AND WORD: the disease correlation with work leave of worker under treatment in the Basic Health Unit

Enfermidade crônica, transmissível e negligenciada, a hanseníase possui indicadores alarmantes e, quando não diagnosticada e tratada precocemente, leva a ocorrência de incapacidades físicas permanentes. Somadas às tantas complicações possíveis da hanseníase, as incapacidades podem interferir de maneira significativa na vida do indivíduo, prejudicando o desenvolvimento de suas atividades do dia a dia, como as laborativas. A redução da capacidade para o trabalho, causada pela doença, compromete a produtividade, podendo levar a interrupção do trabalho, afastando ou, até mesmo, excluindo definitivamente indivíduos da cadeia produtiva. O afastamento do trabalho por motivo de doença possui repercussões financeiras e psicossociais ao trabalhador. Esse estudo objetivou analisar a correlação da hanseníase com o afastamento do trabalhador que realiza tratamento na Unidade Básica de Saúde. Trata-se de um estudo transversal, analítico com abordagem quantitativa, realizado em 10 unidades de saúde notificadoras de hanseníase do município de Belém-Pará. Foram selecionados 72 pacientes com diagnóstico de hanseníase, com idade igual ou superior a 18 anos e que possuíam alguma atividade trabalhista, independente do vínculo empregatício. A amostra foi composta por trabalhadores com média de idade de 42,9 anos, 69,4% do sexo masculino, 45,8% com nível de escolaridade fundamental, renda mensal de 1 a 3 salários mínimo (59,7%), ocupando cargos de trabalhadores dos serviços, vendedores do comércio em lojas e mercados (31,9%). Foi evidenciada predominância da forma clínica Dimorfa (72,2%), sem nervos afetados (52,8%) e, com 5 a 10 lesões cutâneas (51,3%). Foi identificada presença de incapacidades físicas em 34,7% dos trabalhadores, sendo esta, a principal causa indicada para os afastamentos do trabalho, problema referido por 59,7% dos trabalhadores. Outros problemas relacionados aos afastamentos do trabalho, indicados pelos trabalhadores foram: restrição ao trabalho (56,9%), redução da capacidade laborativa (55,5%), omissão do diagnóstico da doença (43%), falta de readaptação/mudança de função (90,2%) e preconceito (26,3%). O tempo de afastamento do trabalho em decorrência da hanseníase foi superior a 6 meses em 48,8% dos casos. Na análise bivariada identificou-se relação estatística com o afastamento do trabalho entre: redução da capacidade laborativa (p< 0.000), restrição ao trabalho (p=0.0001), maior número de nervos afetados (p=0.0015), presença de episódios reacionais (p=0.0086). Percebeu-se que, os afastamentos do trabalho estiveram relacionados, com maior grau de significância, às formas multibacilares da doença, aos trabalhadores com baixo índice de escolaridade, ocupando funções que exigem baixo ou nenhum conhecimento técnico, no setor informal de trabalho. Conclui-se que existe relação significativa entre a hanseníase e o afastamento do trabalho do paciente em acompanhamento na Unidade Básica de Saúde e que, a presença de incapacidades físicas, é considerada a principal causa de afastamento do trabalhador. É necessária maior articulação entre as estratégias de atenção à hanseníase e a saúde do trabalhador para a proposição de medidas que visem a minimização de afastamentos do trabalho em decorrência da doença

Chronic, transmissible and neglected disease, leprosy has alarming indicators and, when not diagnosed and treated early, leads to the occurrence of permanent physical disabilities. In addition to the many possible complications of leprosy, disabilities can significantly interfere with the individual's life, hampering the development of their day-to-day activities, such as work activities. The reduction of work capacity, caused by the disease, compromises productivity, which can lead to interruption of work, distancing or even definitively excluding individuals from the productive chain. The work leave due to illness has financial and psychosocial repercussions on the worker. This study aimed to analyze the correlation of leprosy with the removal of the worker who is being treated at the Basic Health Unit. This is a transversal, analytical study with a quantitative approach carried out in ten health units reporting leprosy in the city of Belém - Pará. Were selected 72 patients with a diagnosis of leprosy, aged 18 years or more and who had some labor activity, regardless of the employment relationship. The sample consisted of workers with a mean age of 42.9 years, 69.4% male, 45.8% with a primary schooling level, monthly income of 1 to 3 minimum wages (59.7%), occupying positions of service workers, store sellers and markets (31.9%). It was evidenced predominance of the clinical form Dimorfa (72.2%), without affected nerves (52.8%) and with 5 to 10 cutaneous lesions (51.3%). The presence of physical disabilities was identified in 34.7% of the workers, being this the main cause indicated for work leave, a problem referred by 59.7% of the workers. Other problems related to work leave, indicated by workers were: work restriction (56.9%), reduction of work capacity (55.5%), omission of the diagnosis of the disease (43%), lack of readaptation change of function (90.2%) and prejudice (26.3%). The time to leave work due to leprosy was greater than 6 months in 48.8% of the cases. In the bivariate analysis we identified a statistical relationship with the work away from: reduction of labor capacity (p <0.000), work restriction (p = 0.0001), higher number of nerves affected (p = 0.0015), presence of reactive? episodes (p = 0.0086). It was observed that the work leave was related, with greater degree of significance, to multibacillary forms of the disease, to workers with low educational level, occupying functions that require little or no technical knowledge in the informal work sector. It is concluded that there is a significant relationship between leprosy and the patient's absence from work in the Basic Health Unit and that the presence of physical disabilities is considered the main cause of the worker's absence. Greater articulation between the strategies of attention to leprosy and the health of the worker is necessary for the proposition of measures that aim at the minimization of absences from work due to the disease

Electrochemical biosensor made with tyrosinase immobilized in a matrix of nanodiamonds and potato starch for detecting phenolic compounds.

The envisaged ubiquitous sensing and biosensing for varied applications has motivated materials development toward low cost, biocompatible platforms. In this paper, we demonstrate that carbon nanodiamonds (NDs) can be combined with potato starch (PS) and be deposited on a glassy carbon electrode (GCE) in the form of a homogeneous, rough film, with electroanalytical performance tuned by varying the relative ND-PS concentration. As a proof of concept, the ND/PS film served as matrix to immobilize tyrosinase (Tyr) and the resulting Tyr-ND-PS/GCE biosensor was suitable to detect catechol using differential pulse voltammetry with detection limit of 3.9 × 10-7 mol L-1 in the range between 5.0 × 10-6 and 7.4 × 10-4 mol L-1. Catechol could also be detected in river and tap water samples. This high sensitivity, competitive with biosensors made with more sophisticated procedures and materials in the literature, is attributed to the large surface area and conductivity imparted by the small NDs (<5 nm). In addition, the ND-PS matrix may have its use extended to immobilize other enzymes and biomolecules, thus representing a potential biocompatible platform for ubiquitous biosensing.

O rendimento da prova tuberculínica entre comunicantes de portadores de tuberculose pulmonar em Belém-PA / The efficiency of tuberculin test among the contacts of people with pulmonary tuberculosis in Belém-PA

Objetivo: Determinar o rendimento da Proteína Purificada Derivada (PPD) em comunicantes de tuberculose pulmonar ativa. Metodologia: Trata-se de um estudo descritivo, transversal e de abordagem quantitativa realizado em duas Unidades Básicas de Saúde localizadas em Belém-PA. Participaram da pesquisa 42 comunicantes de ambos os sexos. As informações foram colhidas por meio de um formulário estruturado, com perguntas fechadas e finalizadas com a realização da aplicação do PPD. Resultados: O rendimento do PPD apresentou 22 comunicantes (52,3%) positivos, 19 comunicantes (45,2%) negativos e 1 contato (2,3%) não retornou para a avaliação. Conclusão: A pesquisa demonstra que a maior parte dos comunicantes que realizam a prova tuberculínica podem estar infectados pelo Bacilo de Koch.

Objective: To determine the efificiency of Derived Purified Protein (PPD) in active pulmonary TB patients. Methodology: This is a descriptive, cross-sectional study with a quantitative approach carried out in two Basic Health Units located in Belém-PA. Participated in the scientific study 42 contacts of both sexes, which the information was collected through a structured form, with closed questions and finalized with the implementation of the PPD. Results: The PPD efficiency presented 22 positive (52.3%) communicants, 19 negative (45.2%) and 1 contact (2.3%) did not return to the evaluation. Conclusion: Research shows that most contacts who perform the tuberculin test may be infected by Koch bacillus.

Objetivo: Determinar el rendimiento de la Proteína Purificada Derivada (PPD) en comunicantes de tuberculosis pulmonar activa. Metodología: Se trata de un estudio descriptivo, transversal y de abordaje cuantitativo realizado en dos Unidades Básicas de Salud ubicadas en Belém-PA. Participaron de la encuesta 42 comunicantes de ambos sexos. La información fue recogida a través de un formulario estructurado, con preguntas cerradas y finalizadas con la aplicación del PPD. Resultados: El ingreso del PPD presentó 22 comunicantes (52,3%) positivos, 19 comunicantes (45,2%) negativos y 1 contacto (2,3%) no regresó para la evaluación. Conclusión: La investigación demuestra que la mayor parte de los comunicantes que realizan la prueba tuberculínica pueden estar infectados por el Bacilo de Koch.

Importância da Residência em Enfermagem no Processo Ensino-Aprendizagem: uma Revisão Integrativa / Importance of Nursing Residency in the Teaching-Learning Process: An Integrative Review

O objetivo desse estudo é analisar as produções científicas dos anos de 2012 a 2016, referentes à importância dos programas de residência para o processo ensino-aprendizagem em enfermagem. Trata-se de uma revisão integrativa da literatura, realizada por meio da Biblioteca Virtual da Saúde (BVS) utilizando as Bases de Dados,LILACS, BDENF e a biblioteca eletrônica SCIELO, utilizando os Descritores em Ciências da Saúde (DeCS):"Internato e Residência", "Preceptoria e Educação em Enfermagem".Foram encontrados 62artigos, dos quais 46foram eliminados conforme critérios de exclusão,restando 16artigospara amostra final, cuja maioria são qualitativos com nível 6 de evidência.A enfermagem tem produzido número significativo de estudos acerca do tema, porém com baixos níveis de evidência. A residência é capaz de propiciar maior segurança ao enfermeiro, suprindo lacunas da graduação e proporcionar melhorias no atendimento ao paciente, no entanto, o processo ensino-aprendizagem requer aperfeiçoamento e investimentos nos enfermeiros preceptores

Objectives: To analyze the scientific productions of the last five years regarding the importance of nursing residency in the teaching-learning process. Method: it is an integrative review of the literature, carried out through the Virtual Health Library (VHL) using the Databases: LILACS, BDENF and the electronic library SCIELO in the period from 2012 to 2016, using the Descriptors in Sciences of the Health (DeCS): Internship and Residence, Preceptory and Nursing Education. Results: 62 articles were found, of which 46 were eliminated according to exclusion criteria, remaining 16 articles for final sample, most of which are qualitative with level 6 of evidence. Conclusion: Nursing has produced a significant number of studies about the subject, but with low levels of evidence. The residency is able to provide greater safety to the nurse, filling gaps in the graduation and provide improvements in patient care, however, the teaching-learning process requires improvement and investments in nurses preceptors